 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Cytotoxicity of Crude Proanthocyanidin Extract from Purple Glutinous Rice Bran (Oryza sativa L.) (Kum Doi Saket) Compared with Cyanidin 3-Glucoside on X63 Myeloma Cancer Cel ผู้เขียน: Montri Punyatong, Puntipa Pongpiachan, Petai Pongpiachan, Dumnern Karladee, Samlee Mankhetkorn สื่อสิ่งพิมพ์:pdf AbstractProanthocyanidin and other phenolic compounds may potentially reduce the risk of cardiovascular diseases and cancer, as well as having antioxidant, anti-inflammatory and chemoprotective properties. Proanthocyanidin (PA) and cyanidin 3-glucoside (C3G), a polyphenolic compound of purple color found in purple glutinous rice (Oryza sativa L.), may also manifest these positive effects. This research evaluated the effect of PA and C3G on X63, a mouse-plasma cancer cell line of myeloma cells. PA and C3G were extracted from the purple rice bran of a local, Thailand, purple, rice genotype (Kum Doi Saket). The results showed that the amount of C3G extract from the rice genotype was 54.47 mg/ 100 g rice bran. The cytotoxicity of the crude PA extract was demonstrated by a dose-dependent decrease in the percentage cell viability of the control in the PA group. A significant difference (p<0.05) began at 100 ?g/ml and IC50 occurred at 62.29 ?g/ml. The C3G extract also exhibited a dose-dependent decrease, but the significant difference (p<0.05) began at 10 ?M and IC50 occurred at 8.4 ?M. This research demonstrated a dose-related cytotoxic effect on cancer cells by the crude PA and C3G extracts from purple glutinous rice. The results indicated the benefit of the purple rice genotypes as a functional food with potential anticancer properties. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Monoclonal Antibodies Production for Quantification of Ochratoxin A in Feedstuffs by Enzyme-Linked Immunosorbent Assay) ผู้เขียน:Montri Punyatong, Puntipa Pongpiachan, Petai Pongpiachan สื่อสิ่งพิมพ์:pdf AbstractOchratoxin A (OTA), a mycotoxin, is produced from Aspergillus and Penicillium species. It is a nephrotoxin which is very toxic for the kidneys, and normally found in feedstuffs and other feeds. In this study, the monoclonal antibody (MAb) against OTA was produced to determine OTA in feedstuffs. It was found that 3 monoclones (5E7-3F2, 5E7-3G6 and 5E7-G10) reacted with OTA. MAb developed a specific and highly sensitive sandwich ELISA. No difference in quantification in samples was found when compared with a commercial test kit (P>0.05) and 50% binding of standard curve was found at 35 pg/assay. In this study OTA in feedstuff could be detected by MAb, which was self-producing by the ELISA method. |